This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.Content:
Chapter 1 Getting Started (pages 1โ20):
Chapter 2 Basics of Separation (pages 21โ58):
Chapter 3 Detection Sensitivity and Selectivity (pages 59โ99):
Chapter 4 Sample Preparation (pages 100โ173):
Chapter 5 The Column (pages 174โ232):
Chapter 6 Non?Ionic Samples: Reversed? and Normal?Phase HPLC (pages 233โ291):
Chapter 7 Ionic Samples: Reversed?Phase, Ion?Pair, and Ion?Exchange HPLC (pages 292โ349):
Chapter 8 Gradient Elution (pages 350โ401):
Chapter 9 Systematic Approach to the Reversed?Phase Separation of Regular Samples (pages 402โ438):
Chapter 10 Computer?Assisted Method Development (pages 439โ478):
Chapter 11 Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds (pages 479โ536):
Chapter 12 Chiral Separations (pages 537โ615):
Chapter 13 Preparative HPLC Separation (pages 616โ642):
Chapter 14 Quantitation (Including Trace Analysis) (pages 643โ684):
Chapter 15 Completing the Method: Validation and Transfer (pages 685โ713):