Practical High-Performance Liquid Chromatography, Fifth Edition

✍ Scribed by Veronika R. Meyer(auth.)

• Year: 2010
• Tongue: English
• Leaves: 411
• Category: Library

✦ Synopsis

Jump into the HPLC adventure!

Three decades on from publication of the 1^st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners.

New sections on the following topics have been included in this fifth edition:

• Comparison of HPLC with capillary electrophoresis
• How to obtain peak capacity
• van Deemter curves and other coherences
• Hydrophilic interaction chromatography
• Method transfer
• Comprehensive two-dimensional HPLC
• Fast separations at 1000 bar
• HPLC with superheated water

In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added.

A completely new chapter is presented on quality assurance covering:

• Is it worth the effort?
• Verification with a second method
• Method validation
• Standard operating procedures
• Measurement uncertainty
• Qualifications, instrument test, and system suitability test
• The quest for quality

Reviews of earlier editions

"That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst

“…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction." - American Journal of Therapeutics

Content:
Chapter 1 Introduction (pages 5–16):
Chapter 2 Theoretical Principles (pages 17–57):
Chapter 3 Pumps (pages 59–64):
Chapter 4 Preparation of Equipment up to Sample Injection (pages 65–79):
Chapter 5 Solvent Properties (pages 81–89):
Chapter 6 Detectors (pages 91–115):
Chapter 7 Columns and Stationary Phases (pages 117–140):
Chapter 8 HPLC Column Tests (pages 141–158):
Chapter 9 Adsorption Chromatography: Normal?Phase Chromatography (pages 159–172):
Chapter 10 Reversed?Phase Chromatography (pages 173–193):
Chapter 11 Chromatography with Chemically Bonded Phases (pages 195–201):
Chapter 12 Ion?Exchange Chromatography (pages 203–215):
Chapter 13 Ion?Pair Chromatography (pages 217–223):
Chapter 14 Ion Chromatography (pages 225–230):
Chapter 15 Size?Exclusion Chromatography (pages 231–247):
Chapter 16 Affinity Chromatography (pages 249–254):
Chapter 17 Choice of Method (pages 255–262):
Chapter 18 Solving the Elution Problem (pages 263–284):
Chapter 19 Analytical HPLC (pages 285–310):
Chapter 20 Quality Assurance (pages 311–320):
Chapter 21 Preparative HPLC (pages 321–332):
Chapter 22 Separation of Enantiomers (pages 333–347):
Chapter 23 Special Possibilities (pages 349–362):
Chapter 24 Appendix 1: Applied HPLC Theory (pages 363–372):
Chapter 25 Appendix 2: How to Perform the Instrument Test (pages 373–385):
Chapter 26 Appendix 3: Troubleshooting (pages 387–395):
Chapter 27 Appendix 4: Column Packing (pages 397–400):