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Practical considerations in refolding proteins from inclusion bodies

✍ Scribed by Kouhei Tsumoto; Daisuke Ejima; Izumi Kumagai; Tsutomu Arakawa

Book ID
117702635
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
172 KB
Volume
28
Category
Article
ISSN
1046-5928

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Overexpression of cloned or synthetic genes in Escherichia coli often results in the formation of insoluble protein inclusion bodies. Within the last decade, specific methods and strategies have been developed for preparing active recombinant proteins from these inclusion bodies. Usually, the inclus