PPRAS1PPRAS2ANDPPRAP1GENES, MEMBERS OF ARASGENE FAMILY FROM THE TRUE SLIME MOLDPHYSARUM POLYCEPHALUMARE DEVELOPMENTALLY REGULATED
✍ Scribed by PIOTR KOZLOWSKI; JOANNA TRZCINSKA-DANIELEWICZ; AGNIESZKA URBANCZYK; KAZIMIERZ TOCZKO
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 116 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
Abstract
The expression patterns of two true__ras__genes,__Ppras1__and__Ppras2__and one__rap__gene,__Pprap1__were examined in four__Physarum polycephalum__developmental stages: uninucleate amoebae, plasmodia (multinucleate syncytia), spherules (a vegetative, dormant stage) and fruiting bodies.Ppras1__and__Pprap1__are expressed in all stages examined with the maximum levels of their transcripts in amoebae and fruiting bodies, respectively, and the minimum levels in plasmodia, whereas the__Ppras2__transcript is only detectable in amoebae and fruiting bodies. The results obtained indicate that__P. polycephalum__is an organism possessing a developmentally regulated__ras__gene family and presents a convenient system to study the role of__ras/__rap__genes in control of growth and differentiation of lower eukaryotic organisms.