Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase

✍ Scribed by A. K. Chen; J. A. Starzmann; C. C. Liu

• Book ID: 101726670
• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 255 KB
• Volume: 24
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260240416

✦ Synopsis

Abstract

Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD^+^ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10^−4^M to 10^−1^M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.