𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Potentiometric determination of coenzyme-dependent oxidoreductase enzymes with recycling of the coenzyme

✍ Scribed by Thomas C. Wallace; Robert W. Coughlin

Book ID
101722927
Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
448 KB
Volume
20
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

By enzymatically establishing a rapid (essentially equilibrium) coupling of a redox coenzyme such as NAD with the components of the ferrocyanide–ferricyanide half‐cell (e.g., using excess diaphorase) the half‐cell potential can be used to monitor another enzymatic reaction involving the same coenzyme. This approach provides a general, rapid potentiometric method of assaying coenzyme‐dependent oxidoreductase enzymes. We show that these assay systems can be designed for multiple turnover of coenzyme (in our case NAD) during a single assay thereby amplifying the rate of electromotive force (emf) change with a concomitant increase in sensitivity of enzyme assay. This allows the use of small concentrations of coenzyme and extension of the range of enzyme concentrations that may be assayed.

📜 SIMILAR VOLUMES

Amperometric assay of coenzyme-dependent
Amperometric assay of coenzyme-dependent oxidoreductase enzymes in a flow-through cell
✍ Thomas C. Wallace; Melonia Berezny Leh; Robert W. Coughlin 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 859 KB

## Abstract We have demonstrated that a simple electrochemical cell can serve as a detector of NADH concentration in a flow system thereby providing an assay technique for NADH dependent enzymes. When this is applied to NADH produced by enzymatic reaction, then a reproducible measure of enzyme acti

The measurement of Coenzyme A and a Coen
The measurement of Coenzyme A and a Coenzyme A-dependent enzyme
✍ H. Hammar; L. Hammar 📂 Article 📅 1979 🏛 Springer-Verlag 🌐 English ⚖ 469 KB

A sensitive micromethod for the determination of Coenzyme A and its esters down to about 0.2 pmol in a volume of 10 pl and of the activity of citrate synthase is outlined. Epidermal material from healthy and psoriatic skin was utilized in pg quantity as tissue source. The assay utilizes the ketoglut