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Potentiometric biosensor for urea based on electropolymerized electroinactive polypyrrole

✍ Scribed by Shinichi Komaba; Michiko Seyama; Toshiyuki Momma; Tetsuya Osaka

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
578 KB
Volume
42
Category
Article
ISSN
0013-4686

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✦ Synopsis

A urea biosensor was developed by immobilizing urease into an electropolymerized electroinactive polypyrrole (PPy) on a platinum electrode. This enzyme-immobilized PPy electrode showed a stable potential response to urea based on the pH response of the electroinactive PPy film electrode. The electropolymerization conditions were optimized, and thus the biosensor showed a Nernstian response with a slope of 31.8 mV decade-' over concentration range of 1 x 10-4-0.3 mol dm-r urea.

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