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Potentiation of the cGMP-induced Guinea pig acrosome reaction by zinc

✍ Scribed by Santos-Sacchi, Joseph ;Gordon, Mildred ;Williams, William L.

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
255 KB
Volume
213
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

The addition of 8 bromo cGMP (10 mM) immediately (∼2 min) upon suspension of Guinea pig sperm in glucose‐free BWW medium induces a significant number of acrosome reactions within minutes. The simultaneous addition of micromolar concentrations of ZnCl~2~ (25–1000 μM) potentiates the cGMP effects. Mid‐micromolar concentrations were able to dramatically increase the percentage of acrosome reactions by a factor of 6.5 over 8 bromo cGMP alone. The induction of acrosome reactions was dependent upon external Ca^++^, and it is suggested that in this species Zn^++^ flnctions by affecting cyclic nucleotide metabolism and/or Ca^++^ flux.

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