We have developed a novel bioreactor based on the observation that isolated porcine hepatocytes rapidly and spontaneously aggregate into spheroids under oscillation conditions. The purpose of this study was to characterize the influence of oscillation frequency (0.125 Hz, 0.25 Hz), cell density (1-1
โฆ LIBER โฆ
Potentiality of Immobilized Pig Hepatocyte Spheroids in Bioartificial Liver System
โ Scribed by J.-H. Lee; D.-H. Lee; J.-K. Park; S.-K. Kim; C.H.D. Kwon; S.-K. Lee
- Book ID
- 113935545
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 349 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0041-1345
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We designed a bioartificial liver support system in which encapsulated multicellular spheroids of rat hepatocytes were utilized as a bioreactor in a hollow fiber cartridge. The spheroids, formed in a positively charged polystyrene dish that contained hormonally defined medium, were encapsulated into