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Potential pitfall in the determination of free [Mg2+] by31P NMR when using the β/α-ATP peak height ratio method

✍ Scribed by Stefan Widmaier; Wulf-Ingo Jung; Günther J. Dietze; Otto Lutz

Book ID
110605936
Publisher
Springer
Year
1999
Tongue
English
Weight
364 KB
Volume
9
Category
Article
ISSN
0968-5243

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Potential pitfall in the determination o
Potential pitfall in the determination of free [Mg2+] by 31P NMR when using the β/α-ATP peak height ratio method
✍ Widmaier, S 📂 Article 📅 1999 🏛 Springer 🌐 English ⚖ 121 KB

Recently, Clarke et al. (Clarke K, Kashiwaya Y, King MT, Gates D, Keon CA, Cross HR, Radda GK, Veech RL. The b/a peak height ratio of ATP. A measure of free [Mg 2 + free ] using 31 P NMR, J. Biol. Chem. 1996;271:21142-21150.) reported a new method to noninvasively determine the concentration of intr

Using the β/α peak-height ratio of ATP i
Using the β/α peak-height ratio of ATP in 31P NMR spectra to measure free [Mg2+]: theoretical and practical problems
✍ Peter J. Mulquiney; Philip W. Kuchel 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 62 KB 👁 1 views

A recently described alternative method for determining free [Mg 2 ] in cells and tissue with 31 P NMR spectroscopy, which is based on the peak-height ratio of the b and a resonances of ATP (h b/a ) [J. Biol. Chem. 271, 21141-21150 (1996)], was found to have numerous problems. These problems include