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Potential of cell retention techniques for large-scale high-density perfusion culture of suspended mammalian cells

✍ Scribed by D. Voisard; F. Meuwly; P.-A. Ruffieux; G. Baer; A. Kadouri


Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
345 KB
Volume
82
Category
Article
ISSN
0006-3592

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## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p