Large-scale production of endotoxin-free
Large-scale production of endotoxin-free plasmids for transient expression in mammalian cell culture
β
Aleksei Rozkov; Bert Larsson; Stefan GillstrΓΆm; Robert BjΓΆrnestedt; Stefan R. Sc
π
Article
π
2007
π
John Wiley and Sons
π
English
β 219 KB
π 1 views
## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100βL scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p