Potent Triple Helix Stabilization by 5′,3′-Modified Triplex-Forming Oligonucleotides
✍ Scribed by Nouha Ben Gaied; Zhengyun Zhao; Simon R. Gerrard; Keith R. Fox; Tom Brown
- Book ID
- 101820711
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 362 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1439-4227
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✦ Synopsis
Abstract
Anthraquinone and pyrene analogues attached to the 3′ and/or 5′ termini of triplex‐forming oligonucleotides (TFOs) by various linkers increased the stability of parallel triple helices. The modifications are simple to synthesize and can be introduced during standard solid‐phase oligonucleotide synthesis. Potent triplex stability was achieved by using doubly modified TFOs, which in the most favourable cases gave an increase in melting temperature of 30 °C over the unmodified counterparts and maintained their selectivity for the correct target duplex. Such TFOs can produce triplexes with melting temperatures of 40 °C at pH 7 even though they do not contain any triplexstabilizing base analogues. These studies have implications for the design of triplex‐forming oligonucleotides for use in biology and nanotechnology.