Posttranslational Modification of Proteins Volume 194 || Assignment of Disulfide Bonds in Proteins by Chemical Cleavage and Peptide Mapping by Mass Spectrometry
โ Scribed by Kannicht, Christoph
- Book ID
- 121466785
- Publisher
- Humana Press
- Year
- 2002
- Weight
- 368 KB
- Category
- Article
- ISBN-13
- 9781592591817
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Intrinsic membrane proteins pose substantial obstacles to analysis by common analytical techniques due to their hydrophobic nature and solubilization requirements. This is the case for studies involving HPLC coupled to mass spectrometry. We have developed an HPLC/mass spectrometry approach to explor
Tandem mass spectrometry employing high-energy, collisionally activated dissociation (CAD) is shown to be a useful method for sequencing through the cystine bridge of intermolecularly disulfide-bonded peptides. A characteristic triplet of intense fragment ions is observed corresponding to cleavage t