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Posttranslational modification of ornithine decarboxylase by its product putrescine

✍ Scribed by Diane Haddock Russell


Book ID
118315399
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
350 KB
Volume
99
Category
Article
ISSN
0006-291X

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## Abstract The addition of 50 ΞΌM exogenous putrescine to Swiss 3T3 cells during serum stimulation resulted in a sixfold increase in intracellular putrescine content and prevented the rise in ornithine decarboxylase activity normally seen in response to serum. SV3T3 cells had putrescine levels ten

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A simple and direct method for the detection of ornithine decarboxylase (EC 4.1.1.17) activity is presented. It is based upon the selective binding of putrescine to Whatman P81 paper, a strong cation-exchanger, in the presence of 0.1 M NH,. The assay is easy to perform and has an advantage over the