Posttranslational modification of ornithine decarboxylase by its product putrescine
β Scribed by Diane Haddock Russell
- Book ID
- 118315399
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 350 KB
- Volume
- 99
- Category
- Article
- ISSN
- 0006-291X
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## Abstract The addition of 50 ΞΌM exogenous putrescine to Swiss 3T3 cells during serum stimulation resulted in a sixfold increase in intracellular putrescine content and prevented the rise in ornithine decarboxylase activity normally seen in response to serum. SV3T3 cells had putrescine levels ten
## Abstract Conditions have been established under which the antizyme of ornithine decarboxylase (E.C. 4.1.1.17, Lβornithine carboxyβlyase, ODC) a noncompetitive protein inhibitor of ODC, can be detected in cells in response to as little as 10^β7^ M putrescine. The maintenance of intracellular anti
A simple and direct method for the detection of ornithine decarboxylase (EC 4.1.1.17) activity is presented. It is based upon the selective binding of putrescine to Whatman P81 paper, a strong cation-exchanger, in the presence of 0.1 M NH,. The assay is easy to perform and has an advantage over the