Postinductive actinomycin D effects on the concentrations of cadmium thionein, and copper chelatin in rat liver

✍ Scribed by Frances A. Day; Barbara J. Coles; Frank O. Brady

• Publisher: Elsevier Science
• Year: 1978
• Weight: 767 KB
• Volume: 8
• Category: Article
• ISSN: 0006-3061
• DOI: 10.1016/s0006-3061(00)80236-7

✦ Synopsis

The time courses of induction in rat liver of copper chelatin by copper, cadmium thionein by cadmium, and zinc thionein by copper, cadmium, and zinc were monitored following single intraperitoneal injections of metal salts. Low dosages of inducing metal were used in order to avoid toxic effects, being 5 mg zinc, 0.5 mg 'copper, and 0.25 mg cadmium per kg body weight. Peak times of induction and half times of decay observed were: copper chelatm (9 h, 8.6 h), cadmium thionein (18 h, 6.80 days), and zinc thionein (zinc rats, 18 h. 10.1 h; copper rats, 9 h, 18.2 h; cadmium rats, 24 h, 4.53 days). Administration of actinomycin D (1 mg per kg body weight) at the peak times of INTRODUCIION Nearly twenty years ago Margosbes and Vallee [l] described the isolation from equine kidney cortex of a cadmium binding, low molecular .weight protein. Because of its rich cysteine and metal content this protein was subsequently named metallothionein 12, 31. Since those early reports, metallothionein has been purified to homogeneity from a variety of sources: human liver [43 and kidney [S], equine liver and kidney [6], rabbit liver [7] and kidney [S], chicken [9], pig [lo], rat 111, 121, and calf and sheep liver [13] _ Although originally isolated with cadmium as the bound metal, metallothionein has been