Post-Translational Modification of Proteins: Tools for Functional Proteomics

✍ Scribed by Christoph Kannicht

• Publisher: Springer New York; Humana
• Year: 2019
• Tongue: English
• Leaves: 331
• Series: Methods in Molecular Biology 1934
• Edition: 3rd ed.
• Category: Library

✦ Synopsis

The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting edge, Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.

✦ Table of Contents

Front Matter ....Pages i-xi
Web-Based Computational Tools for the Prediction and Analysis of Posttranslational Modifications of Proteins (Vladimir A. Ivanisenko, Timofey V. Ivanisenko, Olga V. Saik, Pavel S. Demenkov, Dmitry A. Afonnikov, Nikolay A. Kolchanov)....Pages 1-20
Detection of Posttranslational Modifications by Fluorescent Staining of Two-Dimensional Gels (Archana M. Jastorff, Chris W. Turck)....Pages 21-32
Analysis of Ser/Thr-Linked Sugar Chains (Kalyan R. Anumula)....Pages 33-42
Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling (Detlef Grunow, Véronique Blanchard)....Pages 43-49
Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry: Detection of Lewis X Structures (Christoph Kannicht, Detlef Grunow, Lothar Lucka)....Pages 51-64
Chromatographic Profiling of N-Glycans (Martin Gohlke, Véronique Blanchard)....Pages 65-81
Activity Determination of Glycosyltransferases (Kalyan R. Anumula)....Pages 83-92
Characterization of Site-Specific N-Glycosylation (Helga Hevér, Zsuzsanna Darula, Katalin F. Medzihradszky)....Pages 93-125
Glycosylation of Type I Collagen (Mitsuo Yamauchi, Marnisa Sricholpech, Masahiko Terajima, Kenneth B. Tomer, Irina Perdivara)....Pages 127-144
Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa (Nahid Azzouz, Peter Gerold, Ralph T. Schwarz)....Pages 145-162
Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods (Christof Lenz)....Pages 163-178
Mass Spectrometry-Based Method for Detection and Identification of Free Thiol Groups in Proteins (Barbara A. Solecka-Witulska, Christoph Weise, Christoph Kannicht)....Pages 179-189
Mass Spectrometric Determination of Protein Ubiquitination (Carol E. Parker, Maria R. E. Warren Hines, Viorel Mocanu, Susanna F. Greer, Christoph H. Borchers)....Pages 191-221
Analysis of Sumoylation (Jan Breucker, Andrea Pichler)....Pages 223-233
An Approach for the Identification of Proteins Modified with ISG15 (Tomoharu Takeuchi, Shun Koinuma, Hideyoshi Yokosawa, Yoichiro Arata)....Pages 235-246
α-Amidated Peptides: Approaches for Analysis (Gregory P. Mueller, Rachel C. Lazarus, William J. Driscoll)....Pages 247-264
S-Acylation of Proteins (Larisa Kordyukova, Ludwig Krabben, Marina Serebryakova, Michael Veit)....Pages 265-291
Analysis of Methylation, Acetylation, and Other Modifications in Bacterial Ribosomal Proteins (Randy J. Arnold, Suraj Saraswat, James P. Reilly)....Pages 293-307
Lysine Hydroxylation and Cross-Linking of Collagen (Mitsuo Yamauchi, Masahiko Terajima, Masashi Shiiba)....Pages 309-324
Back Matter ....Pages 325-327

✦ Subjects

Life Sciences; Posttranslational Modification; Protein Science