Post mortem Release of Fish White Muscle α-Actinin as a Marker of Disorganisation
✍ Scribed by Papa, Iris; Alvarez, Cristina; Verrez-Bagnis, Véronique; Fleurence, Joël; Benyamin, Yves
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 697 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
a-Actinin release and its degradation from myofibrils Z-line were studied in post mortem white dorsal muscle from bass and sea trout stored at 4°C and 10°C. Using a-actinin specific antibodies, we show that this protein is rapidly released within the first 24 h for the two species, and reaches a plateau within 4 days. Proteolysis take place very rapidly in bass muscle yielding 80 and 40 kDa fragments from a-actinin as major bands of proteolysis. Sea trout muscle is more resistant, and muscle stored at 4°C is not significantly a-actinin degraded even 10 days after death. In the case of sea trout muscle stored at 10"C, an increasing quantity of 80 and 40 kDa fragment can be observed after the third day. These results show that release and proteolysis of a-actinin are time-and temperaturedependent processes that take place at the early stages of fish storage. Furthermore, we observed that proteolysis of a-actinin seems to be dependent on fish species. In both species studied, the early release of a-actinin comes before the degradation of released molecules, and appears as a biphasic process throughout the disorganisation of post mortem muscle in fish cold-stored above 0°C.