Possible function of tRNAACGThr in regulation of solvent formation in Clostridium acetobutylicum
✍ Scribed by Uwe Sauer; Peter Dürre
- Book ID
- 109318081
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 534 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0378-1097
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The genes coding for enzymes involved in butanol or butyrate formation were subcloned into a novel Escherichia coli-Clostridium acetobutylicum shuttle vector constructed from pIMP1 and a chloramphenicol acetyl transferase gene. The resulting replicative plasmids, referred to as pTHAAD (aldehyde/alco
Metabolic flux analysis was used to investigate the roles of the acid formation pathways in Clostridium acetobutylicum. The acid formation pathways were revealed to serve different roles in wildtype fermentations than previously expected. Specifically, enzymes known to catalyze butyrate formation we