Possible contribution of CD44 variant 6 and nuclear β-catenin expression to the formation of budding tumor cells in patients with T1 colorectal carcinoma
✍ Scribed by Tadahiko Masaki; Akiteru Goto; Masanori Sugiyama; Hiroyoshi Matsuoka; Nobutsugu Abe; Atsuhiko Sakamoto; Yutaka Atomi
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 487 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
In an earlier study, the authors demonstrated that tumor budding was useful for predicting lymph node metastasis in patients with early invasive (T1) colorectal carcinoma. This study was undertaken to clarify the associations between tumor budding, E-cadherin-catenin complex, and CD44 variant 6 abnormalities.
METHODS.
In 51 T1 colorectal carcinomas, tumor budding (the number of dedifferentiation units at the invasive margin) on hematoxylin and eosin-stained slides was counted under light microscopy. Immunostaining for E-cadherin, ␣-catenin, -catenin, and CD44 variant 6 was performed on formalin fixed, paraffin embedded sections. The associations between locoregional failure (lymph node metastasis or local recurrence) and tumor budding and clinicopathologic parameters and immunoreactivity were examined statistically.