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Positive cooperativity in the porcine carboxypeptidase B-catalyzed hydrolysis of neutral peptide substrates

โœ Scribed by Graham J. Moore; N. Leo Benoiton

Book ID
118853628
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
348 KB
Volume
47
Category
Article
ISSN
0006-291X

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โœ Abdulkader Rahmo; Thomas H. Fife ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 151 KB

N-benzoyl-L-phenylalanyl-L-phenylalanine is an excellent peptide substrate for carboxypeptidase A; at 30ยฐC and pH 7.5, K m is 2.6 ร— 10 -5 M while k cat is 177 s -1 (k cat /K m = 6.8 ร— 10 6 M -1 s -1 ). Indole-3-acetic acid is a noncompetitive or mixed inhibitor towards the peptide and toward hippury