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Positive and negative ion matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of complex glycans released from hen ovalbumin and derivatized with­2-aminoacridone

✍ Scribed by Simon North; Helen Birrell; Patrick Camilleri

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
203 KB
Volume
12
Category
Article
ISSN
0951-4198

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✦ Synopsis

2-Aminoacridone derivatives of complex glycans, released from hen ovalbumin, have been chromatographed on a hydrophilic interaction column and fractions collected for analysis by a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer operated in the positive and negative ion modes. Such flexibility in detection has proved to be very useful in the preliminary characterization of a wide range of neutral and sialylated oligosaccharides covalently bound to this glycoprotein.

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