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Porous substances immobilizing enzymes with polymer matrix

✍ Scribed by Minoru Kumakura; Isao Kaetsu

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
401 KB
Volume
37
Category
Article
ISSN
0021-8995

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✦ Synopsis

The preparation of porous substances immobilizing enzymes with polymer matrix having various properties have been studied by radiation polymerization method. The enzyme (cellulase) was immobilized on the surface parts of porous substances such as activated carbon, molecular sieve, silica gel, and coating with hydrophilic and hydrophobic monomers. As substrate of enzyme reaction, lignocellulosic wastes such as chaff pretreated by radiation irradiation (100 Mrad) were used. The enzyme activity of the immobilized enzyme substances was markedly affected by hydrophilicity of monomer and the copolymerization of methoxypoly(ethy1e glycol) methacrylate (75%) and poly(ethy1ene glycol) dimethacrylate (25%) monomer gave the most highest enzyme activity. The immobilized enzyme substances was able to hydrolyze chaff pretreated by radiation irradiation.

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