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Porous polydimethylsiloxane membranes for enzyme immobilization

✍ Scribed by Hong Ying Wang; Takaomi Kobayashi; Hitoshi Saitoh; Nobuyuki Fujii

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
779 KB
Volume
60
Category
Article
ISSN
0021-8995

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✦ Synopsis

SYNOPSIS

Porous polydimethylsiloxane (PDMS) membranes with a-amylase or glucose oxidase activity were prepared by catalytic hydrosilylation cure of PDMS in the presence of the enzymewater solution. The pores in the membrane are the result of hydrogen foams, which are generated during the curing reaction. The enzyme reactions were examined in batch and permeation experiments by using glucose and starch solutions as substrates. For the permeation set-up, the reaction yields of the immobilized a-amylase increased as the permeation rate of the starch solution decreased. The Michaelis-Menten type of reaction kinetics for the immobilized enzyme indicated that the permeation system is effective for the diffusion through the solute of the matrix, as compared with the batch system. 0 1996 John Wiley & Sons, Inc.

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