Porous PEOT/PBT scaffolds for bone tissue engineering: Preparation, characterization, andin vitro bone marrow cell culturing
✍ Scribed by Claase, Menno B. ;Grijpma, Dirk W. ;Mendes, Sandra C. ;de Bruijn, Joost D. ;Feijen, Jan
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 288 KB
- Volume
- 64A
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Abstract
The preparation, characterization, and in vitro bone marrow cell culturing on porous PEOT/PBT copolymer scaffolds are described. These scaffolds are meant for use in bone tissue engineering. Previous research has shown that PEOT/PBT copolymers showed in vivo degradation, calcification, and bone bonding. Despite this, several of these copolymers do not support bone marrow cell growth in vitro. Surface modification, such as gas‐plasma treatment, is needed to improve the in vitro cell attachment. Porous structures were prepared using a freeze‐drying and a salt‐leaching technique, the latter one resulting in highly porous interconnected structures of large pore size. Gas‐plasma treatment with CO~2~ generated a surface throughout the entire structure that enabled bone marrow cells to attach. The amount of DNA was determined as a measure for the amount of cells present on the scaffolds. No significant effect of pore size on the amount of DNA present was seen for scaffolds with pore sizes between 250–1000 μm. Light microscopy data showed cells in the center of the scaffolds, more cells were observed in the scaffolds of 425–500 μm and 500–710 μm pore size compared to the ones with 250–425 μm and 710–1000 μm pores. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 64A: 291–300, 2003