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Pore kinetics reflected in the dequenching of a lipid vesicle entrapped fluorescent dye

โœ Scribed by Gerhard Schwarz; Anna Arbuzova

Book ID
115731036
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
579 KB
Volume
1239
Category
Article
ISSN
0005-2736

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The fluorescent compound, 4',5'-bis[N,N-bis(carboxymethyl)aminomethyl] fluorescein (calcein) has been characterized for use in lipid vesicle studies. Particularly useful is its reaction with Co2+, which results in fluorescence quenching. This is accompanied by about a 10-nm blue shift in the uv abso