This study aimed at analyzing the regulation of in vitro serotonin expression by neurons taken from different regions of the embryonic rat rhombencephalon. We studied the influence of co-culture with alarplate tissue using immunocytochemical and biochemical methods.
Computer-assisted densitometry was used to estimate the co-culture effects on the serotonin content of the cell bodies. The more dynamic aspects of serotonin expression, such as synthesis and release, were studied by measuring (3H)serotonin newly synthesized from (3H)tryptophan.
The density of the immunostaining was significantly decreased in Bl,B2 cells by co-culture with both caudal and rostral alar-plate tissue. For B4-B9 cells, only co-culture with rostral alar-plate tissue produced a significant decrease. The de novo synthesis of serotonin was significantly decreased in Bl,B2 neurons co-cultured with caudal alar-plate tissue only. Once again, the B k B 9 cells proved to be less influenced by the experimental conditions, as co-culture with both types of alar-plate tissue produced no significant effect.
We concluded that the in vitro expression of serotonin can be modulated by environmental factors, but the relative influence of these factors is very different in rostral versus caudal serotonin expressing cell populations.