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Polythiophene gold nanoparticles composite film for application to glucose sensor

✍ Scribed by Pratibha Pandey; Sunil K. Arya; Zimple Matharu; S. P. Singh; Monika Datta; B. D. Malhotra


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
173 KB
Volume
110
Category
Article
ISSN
0021-8995

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✦ Synopsis


Abstract

Regioregular poly‐3‐hexylthiophene (regP3HT) and dithiobissuccinimidyl propionate (DTSP) have been used to prepare bifunctionalized gold nanoparticles (Bf AuNPs). Processable properties of regP3HT have been used to obtain regP3HT‐AuNPs‐DTSP film on a gold‐coated glass surface and the succinimidyl group of DTSP in this regP3HT‐AuNPs‐DTSP/Au electrode has been utilized for covalent immobilization of glucose oxidase (GOx). The UV‐visible (UV‐vis), scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) spectroscopic studies have been used to characterize regP3HT‐AuNPs‐DTSP/Au and GOx‐regP3HT‐AuNPs‐DTSP/Au electrode, respectively. This GOx‐regP3HT‐AuNPs‐DTSP/Au bioelectrode shows response time of 10 s, linearity from 25 to 300 mg/dL of glucose and the value of Michaelis‐Menten constant as 5.85 m__M__ (105.3 mg/dL). © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008


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## Abstract The direct electrochemistry of glucose oxidase (GOx) immobilized on a composite matrix based on chitosan (CHIT) and Au nanoparticles (Au NP) underlying on a glassy carbon electrode was achieved. The cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize