A n c h o r a g e -d e p e n d e n t m a m m a l i a n cells were cultivated at high cell density in a novel culture system using p o l y u r e t h a n e f o a m ( P U F ) as a s u b s t r a t u m for cell attachment. P U F has a m a c r o p o r o u s structure giving a high surface a r e a to v o l
Polystyrene substratum for bulk culture of anchorage dependent cells
β Scribed by A. Kadouri; D. Sher; N. G. Maroudas
- Book ID
- 104741550
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 968 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0920-9069
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β¦ Synopsis
Twisted ribbons made of polystyrene were used as a packing material for the cultivation of anchorage dependent cells. Normal human fibroblast cells grown on this support in a laboratory scale reactor reached densities of about 5-7Γ10(5) cells/ml. The cells adhered strongly to the carrier and no cell detachment was observed upon transfer to serum free medium. The properties of this packing material and its potential use are discussed.
π SIMILAR VOLUMES
Two methods for growing anchorage-dependent cells were adapted for nuclear magnetic resonance (NMR) measurements: growing cells on agarose polyacrolein microsphere beads and on "filters" made of nonwoven polyester fabric. Both were found to be convenient and most suitable for NMR studies in any conv