Polystyrene-coated micropallets for culture and separation of primary muscle cells
β Scribed by David A. Detwiler; Nicholas C. Dobes; Christopher E. Sims; Joe N. Kornegay; Nancy L. Allbritton
- Book ID
- 105895902
- Publisher
- Springer
- Year
- 2011
- Tongue
- English
- Weight
- 317 KB
- Volume
- 402
- Category
- Article
- ISSN
- 1618-2650
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Vascular smooth muscle cells derived from the rat aorta were cultured on unmodified or F + ion-implanted polystyrene (5 Γ10 12 or 5 Γ10 14 ions/cm 2 , energy 150 keV). In 1-day-old cultures, the cells adhered to the modified polystyrene in higher numbers and over larger contact areas. Increased resi
Primary cardiac cell cultures of newborn rats containing approximately 50% (by cell number) spontaneously contracting cardiomyocytes were used to study the role of protein N-glycosylation for the binding of dihydropyridine (DHP) to the voltage-dependent L-type calcium channel. This binding is not in