Polysaccharide production in Pseudomonas cepacia

✍ Scribed by Dr. David G. Allison; Melanie J. Goldsbrough

• Book ID: 102910404
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 550 KB
• Volume: 34
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620340102

✦ Synopsis

The effects of glucose, osmolarity. temperature and mode of growth on exopolysaccharide production in Pseiru'omonas cepuciu was studied in batch culture using a chemically defined growth medium. Polymer production was maximal under conditions of a 2% (w/v) glucosc supplement. 0.4 M NaCl and an incubation temperature of 35 "C. In addition, polysaccharide composition and molecular weight varied with mode of growth. On agar culture there was a decrease in pyruvate and rhamnose content yct an increase in the amount ofacetatecompared to the polymer isolated from broth culture equivalents. The clincial implications of thcse results are discussed in rclation to thc potential pathogenicity of P. cq7crciu in cystic fibrosis patients.