Polymorphism and chromosomal location of endogenous α-amylase inhibitor genes in common wheat

✍ Scribed by P. Masojć; J. Zawistowski; N. K. Howes; T. Aung; M. D. Gale

• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 992 KB
• Volume: 85
• Category: Article
• ISSN: 0040-5752
• DOI: 10.1007/bf00215045

✦ Synopsis

Polymorphism of an endogenous α-amylase inhibitor in wheat was studied using iso-electric focusing followed by monoclonal antibody - based immunoblotting. Ten isoforms of the inhibitor detected in common wheat and its wild counterparts were assigned to five homoeologous loci. Three α-amylase inhibitor loci (Isa-1) were identified in common wheat and located on the long arms of chromosomes 2A, 2B and 2D. In a sample of 27 bread wheats, eight durum wheats, and 12 diploid wheat relatives, amphiploids and triticales, a high resolution isoelectric-focusing separation demonstrated two active and one null allele at the Isa-A1, two alleles at the Isa-B1, one allele at the Isa-D1, four alleles at the Isa-S1, and one allele at the Isa-G1 locus. The most frequent electrophoretic pattern of common wheat cultivars consisted of two isoforms, encoded respectively by the Isa-B1b, Isa-D1 a alleles and the Isa-Alnull allele. All the durum wheats had only one inhibitor form controlled by allele Isa-B1b, which was accompanied by the null allele at the Isa-A1 locus.