Polymorphic microsatellites developed by cross-species amplifications in common pheasant breeds

✍ Scribed by M. Baratti; A. Alberti; M. Groenen; T. Veenendaal; F. D. Fulgheri

• Book ID: 104457415
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 63 KB
• Volume: 32
• Category: Article
• ISSN: 0268-9146
• DOI: 10.1046/j.1365-2052.2001.00767.x

✦ Synopsis

Genetic variability was analysed in two common breeds of pheasant (Phasianus colchicus L. 1758) by means of cross‐species amplifications of microsatellite loci: 154 chicken, Gallus gallus and 32 turkey, Meleagris gallopavo, primers were tested for amplification of pheasant DNA. Thirty‐six primers (25 specific for chicken and 11 for turkey) amplified pheasant DNA. Fifteen markers yielded specific products and were tested for polymorphism. Eight of them (55%) were polymorphic, with an average polymorphism of two alleles per locus. Specific polymerase chain reaction (PCR) products were sequenced; repeats were found in 11 of the 15 markers, although only two loci showed the same repeat and could be homologous to chicken ones.