Polymer–protein interaction, water retention, and biocompatibility of a stimuli-sensitive superporous hydrogel containing interpenetrating polymer networks
✍ Scribed by Lichen Yin; Ziming Zhao; Yizhe Hu; Jieying Ding; Fuying Cui; Cui Tang; Chunhua Yin
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 440 KB
- Volume
- 108
- Category
- Article
- ISSN
- 0021-8995
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✦ Synopsis
Abstract
The swelling of a superporous hydrogel containing poly(acrylic acid‐co‐acrylamide)/O‐carboxymethyl chitosan interpenetrating polymer networks (SPH‐IPN) was sensitive toward the pH, ionic strength, and temperature stimuli. With insulin as a model drug, polymer–protein interaction was detected, and it was physical rather than covalent. Freezing water was the majority of the imbibed water in the swollen SPH‐IPNs, and the water‐retention ability of the polymer against compression and time of exposure at 37°C was improved as the amount of the O‐carboxymethyl chitosan network increased. A 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay on AD293 and RBL‐2H3 cells and an in situ lactate dehydrogenase assay and morphological study on rat intestine confirmed that the SPH‐IPNs had satisfactory biocompatibility. These pronounced properties suggested that the SPH‐IPNs could be developed as an attractive peroral delivery vehicle for peptide and protein drugs. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008