Polymerase chain reaction-based identification of insecticide resistance genes and DNA methylation in the aphid Myzus persicae (Sulzer)
โ Scribed by L. M. Field; S. E. Crlck; A. L. Devonshire
- Book ID
- 114729429
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 889 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0962-1075
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The peach-potato aphid Myzus persicae (Sulzer) can resist a range of insecticides by over-producing detoxifying esterase and having mutantinsensitive forms of the target proteins, acetylcholinesterase (AChE), and the sodium channel. Using a combination of bioassays, biochemical and DNA diagnostics,
Insecticide sprays were applied to Myzus persicae (Sulzer) populations carrying various combinations of three insecticide resistance mechanisms (esterase-based metabolic resistance and two target site mechanisms, known as MACE and kdr), supported on host plants growing in ยฎeld simulator cages. The s