Polymerase Chain Reaction-based Enzyme Immunoassay for Quantitation of Telomerase Activity: Application to Colorectal Cancers†

A highly sensitive and quantitative telo

A highly sensitive and quantitative telomerase activity assay with pancreatic juice is useful for diagnosis of pancreatic carcinoma without problems due to polymerase chain reaction inhibitors : Analysis of 100 samples of pancreatic juice from consecutive patients

✍ Kenoki Ohuchida; Kazuhiro Mizumoto; Nami Ishikawa; Norihiro Sato; Eishi Nagai; K 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 284 KB

## Abstract ## BACKGROUND Early detection of pancreatic carcinoma is difficult even with current diagnostic tools. Novel biomarkers and detection techniques are urgently needed. Telomerase activity is a promising diagnostic marker. However, the conventional telomeric repeat amplification protocol