Polymer molecular weight characterization by temperature gradient high performance liquid chromatography

✍ Scribed by Hee Cheong Lee; Taihyun Chang

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 382 KB
• Volume: 37
• Category: Article
• ISSN: 0032-3861
• DOI: 10.1016/s0032-3861(96)00510-1

✦ Synopsis

We report a novel method of temperature gradient interaction h.p.1.c. for the characterization of molecular weight distribution of macromolecules. A very fine and reproducible control of interaction between polymer chains and the packing material can be achieved by altering the column temperature. A mixture of 10 polystyrene standard samples (molecular weight range: 1700-2 890 000) of narrow molecular weight distribution are analysed by this method. Near complete separation down to the baseline is achieved with the use of a single Cl8 bonded silica column. This method is thus proven to provide a much superior resolution to the conventional size exclusion chromatography.