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Poly(L-lysyl-L-alanyl-α-L-glutamic acid)

✍ Scribed by H. Joseph Goren; T. Fletcher; R. M. Epand

Publisher
Wiley (John Wiley & Sons)
Year
1977
Tongue
English
Weight
651 KB
Volume
16
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

Poly(Lys(Cbz)‐Ala‐Glu(OBzl)) was prepared by the self‐condensation of Lys(Cbz)‐Ala‐Glu(OBzl)‐ONSu in dimethylformamide. After deprotection of the side chains, the product was subjected to Sephadex G‐50 chromatography. The molecular weight of unfractionated and fractionated poly(Lys‐Ala‐Glu) was calculated from a calibrated Sephadex G‐50 column, spectrophotometrically from Dnp‐(Lys‐Ala‐Glu), equilibrium centrifugation, and viscosity measurements. Approximately 21% of the unfractionated material was polymeric with the remaining 79% being cyclic and monomeric material. Treatment of polymer hydrolysate with L‐amino acid and D‐amino acid oxidase indicated poly(Lys‐Ala‐Glu) to be optically pure. The apparent pKa's of the two ionizable groups were 4.1 and 9.7.

📜 SIMILAR VOLUMES

Poly(L-lysyl-L-alanyl-α-L-glutamic acid)
Poly(L-lysyl-L-alanyl-α-L-glutamic acid). II. Conformational studies
✍ H. Joseph Goren; Carl R. McMillin; Alan G. Walton 📂 Article 📅 1977 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 685 KB

## Abstract The solution characterization of poly(Lys‐Ala‐Glu) is described. This polytripeptide is zwitterionic at neutral pH and is shown to take on a conformation which is dictated by the state of ionization, molecular weight, temperature, and solvent. The polypeptide is almost entirely α‐helica

Viscosity and potentiometric measurement
Viscosity and potentiometric measurements of poly(L-histidyl-L-alanyl-α-L-glutamic acid) and poly(L-lysyl-L-alanyl-α-L-glutamic acid)
✍ H. Joseph Goren; Loretta Grandan; Alfred W. L. Jay; Noah Lotan 📂 Article 📅 1977 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 629 KB

## Abstract Poly(His‐Ala‐Glu) and poly(Lys‐Ala‐Glu) were examined by viscosity and potentiometric titration. These measurements were interpreted in terms of the hydrodynamic size of the above sequential polypeptides. Effects of polymer, size and concentration, and solution‐salt concentration were d

Poly(L-histidyl-L-alanyl-α-L-glutamic ac
Poly(L-histidyl-L-alanyl-α-L-glutamic acid). I. Synthesis
✍ H. Joseph Goren; Ephraim Katchalski-Katzir; Mati Fridkin 📂 Article 📅 1977 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 743 KB

## Abstract Poly(L‐histidyl‐L‐alanyl‐α‐L‐glutamic acid) has been prepared in order to test the acid–base catalytic ability of a carboxyl‐imidazole hydrogen‐bonded system. Two different blocked histidyl‐alanyl‐glutamic acid monomers were used in the polymerization step. The imidazole ring was blocke

Poly(L-histidyl-L-alanyl-α-L-glutamic ac
Poly(L-histidyl-L-alanyl-α-L-glutamic acid). II. Catalysis of p-nitrophenyl acetate hydrolysis
✍ H. Joseph Goren; Thea Fletcher; Mati Fridkin; Ephraim Katchalsi-Katzir 📂 Article 📅 1978 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 643 KB

## Abstract The hydrolysis of __p__‐nitrophenyl acetate is catalyzed by imidazole, free in solution or as the side chain in poly(His‐Ala‐Glu). This is based on the observations that the reaction is first order in ester and first order in nonprotonated imidazole. Catalysis of __p__‐nitrophenyl aceta

Conformational changes of poly(L-histidy
Conformational changes of poly(L-histidyl-L-alanyl-α-L-glutamic acid) in solution. Transition pathways and conformational intermediates
✍ H. Joseph Goren; Mati Fridkin; Ephraim Katchalskikatzir; Noah Lotan 📂 Article 📅 1979 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 597 KB

## Abstract The conformation and conformational transitions of poly(His‐Ala‐Glu) have been investigated by ir, nmr, and CD measurements. The results obtained—as well as the results of our previous investigations by potentiometric titration and hydrodynamic techniques [Goren et al., __Biopolymers__

Block oligopeptides (L-lysyl)m-(L-alanyl
Block oligopeptides (L-lysyl)m-(L-alanyl)L-tyrosyl-(L-alanyl)n- (L-lysyl)m. I. Synthesis through fragment condensation and characterization
✍ R. Mayer; A. Caille; G. Spach 📂 Article 📅 1978 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 608 KB

## Abstract Model peptides containing one aromatic residue were synthesized and characterized in order to investigate their interactions with polynucleotides. Chromatographically pure block oligopeptides (L‐alysyl)~__m__~‐(L‐alanyl)~__n__~‐ L‐tyrosyl‐ (L‐alanyl)~__n__~, with __n__ = 3 and __m__=3 o