Polyinosinic acid: polycytidylic acid promotes T helper type 1-specific immune responses by stimulating macrophage production of interferon-α and interleukin-12
✍ Scribed by Roberto Manetti; Francesco Annunziato; Ljiljana Tomasevic; Valeria Giannò; Paola Parronchi; Sergio Romagnani; Enrico Maggi
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 533 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Polyinosinic acid: polycytidylic acid promotes T helper type 1-specific immune responses by stimulating macrophage production of interferona and interleukin42
The effects exerted on the development in vitro of Derrnatophagoides pteronyssinus group I (Der p 1)-specific T cell lines and T cell clones by addition of polyinosinic acid : polycytidylic acid (poly I : C) in lymphocyte bulk culture were examined. Der p I-specific T cell lines generated in presence of poly I : C exhibited reduced ability to produce interleukin (1L)-4 and IL-5 and developed into Der p I-specific CD4' T cell clones showing a T helper (Th) type 0 or Thl, instead of ThO/Th2 cytokine profile. This effect was prevented by addition to lymphocyte bulk cultiirps of a mixture of antibodies specific for interferon (1FN)-a and IL-12, whereas the addition of anti-IFN-a or anti-IL-12 antibody alone was uneffective. Poly I : C also showed the ability to stimulate the production of noticeable amounts of both IFN-a and IL-12 by human monocytes. Taken together, these data suggest that poly I : C is a Thl-inducing agent whose activity is mediated by its ability to stimulate the production of IFN-a and IL-12 by monocytes.