## Abstract Summary: Eleven laboratoryβevolved polyhydroxyalkanoate (PHA) synthases which originated from __Pseudomonas__ sp. 61β3 enzyme (PhaC1~Ps~), together with the wildβtype enzyme, were applied for PHA synthesis from fructose using __Ralstonia eutropha__ PHB^β^4 as a host strain. The evolved
Polyhydroxyalkanoate (PHA) Homeostasis: The Role of the PHA Synthase
β Scribed by JoAnne Stubbe; Jiamin Tian
- Publisher
- John Wiley and Sons
- Year
- 2003
- Weight
- 49 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0931-7597
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β¦ Synopsis
Abstract
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## Abstract The identification of __phaC__ which encodes PHA synthase, that is involved in the formation of polyhydroxyalkanoate (PHA) containing 3βhydroxyβ2βmethylvalerate (3H2MV), was attempted. As of now, PHA containing 3H2MV has been reported to be produced only by mixed microbial cultures in a
Ralstonia eutropha biopolyester synthase (PhaC Re ) is a key enzyme for catalyzing formation of polyhydroxybutyrate (PHB) or polyhydroxyalkanoate (PHA) copolyesters from (R)-3-hydroxybutyryl-CoA (3HB-CoA) and/or (R)-3-hydroxyalkanoyl-CoA (3HA-CoA). Previously, in Escherichia coli, we found a good co