Formation of tetraploid mouse blastocyst
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Eglitis, Martin A.
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Article
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1980
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John Wiley and Sons
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English
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## Abstract A rapid method to produce tetraploid embryos by blastomere fusion using polyethylene glycol (PEG) has been developed. Individual fourโcell stage blastomeres were aggregated into pairs with phytohemagglutinin (PHA) and then treated with 45% (w/v) PEG (MW 1,000). 56.8% (804/1,416) of trea