Polyelectrolyte microcapsules with entrapped multicellular tumor spheroids as a novel tool to study the effects of photodynamic therapy
✍ Scribed by Daria S. Zaytseva-Zotova; Olga O. Udartseva; Elena R. Andreeva; Artur Bartkowiak; Lina N. Bezdetnaya; François Guillemin; Jean-Louis Goergen; Elena A. Markvicheva
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 504 KB
- Volume
- 97B
- Category
- Article
- ISSN
- 1552-4973
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✦ Synopsis
Abstract
In the current study, semi‐permeable alginate‐oligochitosan microcapsules for multicellular tumor spheroids (MTS) generation were elaborated and tested, to estimate a response of the microencapsulated MTS (MMTS) to photodynamic therapy (PDT). The microcapsules (mean diameter 600 μm) with entrapped human breast adenocarcinoma MCF‐7 cells were obtained using an electrostatic bead generator, and MMTS were generated by in vitro long‐term cell cultivation. The formed MMTS were incubated in Chlorin e6 photosensitizer solution and then irradiated using 650‐nm laser light. The cell viability was measured by MTT‐assay in 24 h after irradiation, and histological analysis was performed. The proposed MTS‐based model was found to be more resistant to the PDT than the two‐dimensional monolayer cell culture model. Thus, MMTS could be considered as a promising three‐dimesional in vitro model to estimate the doses of drugs or parameters for PDT in vitro before carrying out preclinical tests. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.