Polydispersity of celluloses and enzymic degraded celluloses by gel permeation chromatography

✍ Scribed by M. Rinaudo; J.P. Merle

• Publisher: Elsevier Science
• Year: 1970
• Tongue: English
• Weight: 448 KB
• Volume: 6
• Category: Article
• ISSN: 0014-3057
• DOI: 10.1016/0014-3057(70)90182-5

✦ Synopsis

The first part of this paper concerns the influence of nitration; the proposed 64 per cent NO3H/36 per cent AcOH mixture is considered to be the best, particularly with native cellulose of high degree of polymerization (DP,, = 4700); nitration to 13-9-14 per cent N is regularly found for a time of contact of 1.5 hr at 2 ° with the nitration mixture. The weight average mol. wt., the degree of substitution and the polydispersity obtained by GPC in THF have been determined.

In the second part of this work, the enzymic degradation by a cellulasic extract of Basidiomycete is followed during 0-48 hr periods on different types of celluloses. After nitration, the residual cellulose is characterized by its mol. wt. and polydispersity. The rate of enzymic modification is discussed and related to the structure of the substrates.