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POLYCLONAL ANTIBODIES IN AN ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT BACTERIAL SPORES

โœ Scribed by P.M. FOEGEDING; Y.H. CHANG

Book ID
111344102
Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
786 KB
Volume
2
Category
Article
ISSN
1060-3999

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โœ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub