𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Polycation Structure Mediates Expression of Lyophilized Polycation/pDNA Complexes

✍ Scribed by Lewis D. Hahn; Hyunjoon Kong; David J. Mooney

Book ID
102467397
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
252 KB
Volume
10
Category
Article
ISSN
1616-5187

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✦ Synopsis

Abstract

Lyophilization of polycation/pDNA complexes provides stable, long‐term storage of complexes prior to clinical use but also reduces gene delivery efficiency. We examined whether polycation structure mediates effects of lyophilization on gene expression. Linear and branched PEI of the same molecular weight were used as a model system. Interestingly, pDNA/linear PEI complexes led to much smaller effects on gene expression following lyophilization compared with branched PEI complexes. The effect of polycation structure correlated with changes in dissociation ability of pDNA/PEI complexes. These results will be useful for developing new gene delivery vehicles.

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