Abstract
Polyaniline/carbon nanotubes composite (PANIโCNT) electrochemically deposited onto indiumโtinโoxide (ITO) coated glass plate has been utilized for Neisseria gonorrhoeae detection by immobilizing 5โฒโaminoโlabeled Neisseria gonorrhoeae probe (aDNA) using glutaraldehyde as a crossโlinker. PANIโCNT/ITO and aDNAโGluโPANIโCNT/ITO electrodes have been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared (FTโIR) spectroscopy, cyclic voltammetry (CV), and differential pulse voltammetry (DPV). This bioelectrode can be used to detect N. gonorrhoeae using methylene blue (MB) as redox indicator with response time of 60โs and stability of about 75 days when stored under refrigerated conditions. DPV studies reveal that this bioelectrode can detect complementary DNA concentration from 1โรโ10^โ6^โM to 1โรโ10^โ17^โM with detection limit of 1.2โรโ10^โ17^โM. Further, this bioelectrode (aDNAโGluโPANIโCNT/ITO) exhibits specificity toward N. gonorrhoeae species and shows negative response with nonโNeisseria gonorrhoeae Neisseria species (NgNS) and other gram negative bacteria (GNB). Copyright ยฉ 2010 John Wiley & Sons, Ltd.