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Poly(A+)mRNA sequences in free mRNP and polysomes of mouse ascites cells

✍ Scribed by Terence Martin; Michael McMullen; Phillip Shaw

Publisher
Springer
Year
1979
Tongue
English
Weight
469 KB
Volume
5
Category
Article
ISSN
0301-4851

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πŸ“œ SIMILAR VOLUMES

The characterization of free, cytoskelet
The characterization of free, cytoskeletal and membrane-bound polysomes in Krebs II ascites and 3T3 cells
✍ Anni Vedeler; Ian F. Pryme; John E. Hesketh πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 755 KB

Polysomes from Krebs II ascites and 3T3 cells were separated into three populations by using a sequential extraction method. Free polysomes were released by using a combination of low salt (25 mM KCl) and NP-40 detergent in the lysis buffer. The cytoskeletal bound polysomes were subsequently release