Poly(amphiphilic pyrrole)-tyrosinase-peroxidase electrode for amplified flow injection-amperometric detection of phenol

✍ Scribed by Serge Cosnier; Ionel Cătălin Popescu

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 598 KB
• Volume: 319
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00479-3

✦ Synopsis

A new approach to enhance the sensitivity of a tyrosinase based biosensor for the amperometric phenol detection at -0.2 V vs. Ag/AgCl (0.1 M KCl) has been described. The procedure consists of the cooperative functioning of tyrosinase and horseradish peroxidase in the presence of H,O,. Monoenzyme and bienzyme electrodes were elaborated by electrochemical polymerisation of amphiphilic pyrrole-enzyme(s) mixtures previously adsorbed on the electrode surface. Batch and flow injection measurements carried out with these biosensors, show that an amplification phenomenon occurs in the presence of H,O,. The role of peroxidase and tyrosinase in the amplification effect has been studied.