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Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine

✍ Scribed by Wen-Chang Chang; Muh-Lin Lee; Chen-Kung Chou; Sheng-Chung Lee

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 290 KB
Volume: 132
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90018-0

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✦ Synopsis

One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.

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