Poly(ADP-ribose) metabolizing enzymes in nuclei and nucleoli of Tetrahymena Pyriformis
โ Scribed by C. Tsopanakis; J.C. Leer; O.F. Nielsen; E. Gocke; S. Shall; O. Westergaard
- Book ID
- 115909145
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 337 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0014-5793
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๐ SIMILAR VOLUMES
Methods are described for the radiolabeling and determination of NAD+, poly(ADP-ribose), and protein-bound monomers of ADP-ribose in cultured mammalian cells. The adenine nucleotide pools of confluent monolayer cell cultures are radiolabeled using high-specific-activity [3H]adenine. Following any de
## Abstract The effect of nicotinamide on unscheduled DNA synthesis was studied in resting human lymphocytes. In cells treated with UV irradiation or with MNNG, nicotinamide caused a twoโfold stimulation of unscheduled DNA synthesis and retarded the rate of NAD^+^ lowering caused by these treatment