Poly-N-vinyl pyrrolidone chromatography : Effect of pH on elution of bases and a proposed mechanism for the adsorption of purines

✍ Scribed by Therese M. Dougherty; Abner I. Schepartz

• Publisher: Elsevier Science
• Year: 1969
• Tongue: English
• Weight: 304 KB
• Volume: 43
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)99220-3

✦ Synopsis

Poly-N-vinyl pyrrolidone chromatography

Effect of pW on elution of bases and a proposed mechanism for the adsorption of purines

Recently the separation of purine and pyrimidine bases by poly-N-vinyl pyrrolidone (PVP) chromatography was reportedl. The principal mechanism of adsorption was attributed to hydrogen and hydrophobic bonding. Electrolytes tend to strengthen hydrophobic bonds whereas they weaken salt linkages". Adenine in distilled water elutes at approximately 80 ml, while adenine in 0.3 ml saturated (NH,),SO, elutes at 56 ml (I x 39.5 cm column). The elution volume of guanine is also decreased in salt3. These observations seem to indicate that hydrophobic bonds are not of major importance in the adsorption of adenine or guanine to PVP.

Compared with the pyrimidines, adenine and guanine bind tenaciously to PVP. Our present studies, reported below, indicate that hydrogen bonding increases the electrophilic character of the carbonyl carbon of PVP and hence the center of high electron density in adenine and guanine adjacent to the point of hydrogen bonding may also be involved.